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Human IFN-β1a protein, His tag (Animal-Free)

Interferon-Beta 1a (IFN-beta 1a) is a leukocyte interferon, which is a variant of Interferon-beta. IFN-beta 1a is a 19 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.

Product Specifications

Background

Interferon-beta (IFN-β1a), acting via STAT1 and STAT2, is known to upregulate and downregulate a wide variety of genes, most of which are involved in the antiviral immune response. It is a member of TypeI IFNs, which include IFN-α, -β, τ, and-ω. IFN-β plays an important role in inducing non-specific resistance against a broad range of viral infections. It also affects cell proliferation and modulates immune responses

Product Name Alternative

IFNB1, Type I Interferon

Accession Number

P01574

Tag

IFN-β1a

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce apoptosis in HeLa cells.The ED₅₀ for this effect is <15 ng/mL.Measure by its ability to induce cytotoxicity in TF-1 cells.The ED₅₀ for this effect is <0.1 ng/mL.The specific activity of recombinant human IFN beta 1a is approximately >1 x10⁷ IU/ mg.

Molecular Weight

The protein has a calculated MW of 20.84 kDa.The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis) .

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The secreted Human IFN-β1a protein consists of 158 amino acids and has a predicted molecular mass of 20.8 kDa

Formulation

Lyophilized from sterile PBS, pH 8.0

Applications Notes

Human

Other Product Names

IFNB1, Type I Interferon

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

Preparation

E. coli

AA Sequence

Amino acid sequence derived from Human IFN-β1a protein (Met30-Asn187) (P01574) with 6xHis tag at the C-terminus was expressed

Available Sizes

Frequently Asked Questions

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