Human MIF Protein

Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine, existing as a homotrimer in vivo. MIF was originally identified as a T cell derived factor responsible for the inhibition of macrophage migration. However, recently MIF has received much more attention because of its possible roles in angiogenesis and cancer development. The intratumoral expression of MIF is strongly correlated with angiogenic growth factor expression, such as the expression of Interleukin 8 (IL-8) and Vascular Endothelial Growth Factor (VEGF), and with risk of recurrence after resection.

Product Specifications

Background

Product Name Alternative

GIF, GLIF, MMIF

Gene ID

4282

Accession Number

P14174

Tag

HMIF

Type

Cytokines and proteins

Applications

No tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Testing in progress

Molecular Weight

12.4 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 1 EU per μg of the protein as determined by the LAL method

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized recombinant Human MIF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant hMIF consists of 115 amino acids with a Met in N terminal and predicts a molecular mass of 12.4 kDa

Formulation

Lyophilized from PBS, pH 6.5.

Applications Notes

Human

Other Product Names

GIF, GLIF, MMIF

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions