Luminescent Mycoplasma Detection Kit
The Luminescent Mycoplasma Detection Kit utilizes Mycoplasma-specific kinase activity and detects it through a luminescent reaction catalyzed by ATP-dependent luciferase, and determines whether a sample is contaminated with Mycoplasma by comparing the change in ATP content before and after addition of the test reagent. When Mycoplasma is cleaved, it releases a unique kinase that reacts with the substrate and catalyzes the conversion of ADP to ATP.
Product Specifications
Background
Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8μm, some mycoplasma can pass 0.22 μm filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition. Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important.
Tag
Mycoplasma
Type
Cell culture
Applications
Mycoplasma detection
Assay Performance Time
15min
Sample Type
Cell
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Simple operation, fast and time-saving, the whole inspection process only two steps, only 15min. • Continuous and stable luminescence, accurate detection.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stored at -20°C for 12 months, protect from light
Formulation
Liquid solution
Other Product Names
Mycoplasma、Mycoplasma PCR Detection
Recommended Usage
•All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.
Available Sizes
Frequently Asked Questions
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