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Luminescent Mycoplasma Detection Kit

The Luminescent Mycoplasma Detection Kit utilizes Mycoplasma-specific kinase activity and detects it through a luminescent reaction catalyzed by ATP-dependent luciferase, and determines whether a sample is contaminated with Mycoplasma by comparing the change in ATP content before and after addition of the test reagent. When Mycoplasma is cleaved, it releases a unique kinase that reacts with the substrate and catalyzes the conversion of ADP to ATP.

Product Specifications

Background

Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8μm, some mycoplasma can pass 0.22 μm filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition. Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important.

Tag

Mycoplasma

Type

Cell culture

Applications

Mycoplasma detection

Assay Performance Time

15min

Sample Type

Cell

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Simple operation, fast and time-saving, the whole inspection process only two steps, only 15min. • Continuous and stable luminescence, accurate detection.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stored at -20°C for 12 months, protect from light

Formulation

Liquid solution

Other Product Names

Mycoplasma、Mycoplasma PCR Detection

Recommended Usage

•All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.

Available Sizes

Frequently Asked Questions

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