CheKine™ Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit

PEPCK (EC 4.1.1.32) is widely found in animals, plants, microorganisms and cells and catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, a key enzyme in the regulation of gluconeogenic pathways.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Determination of PEPCK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

CheKine™ Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit provides a simple, convenient and rapid method for PEPCK activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma) and other samples. The principle is that PEPCK catalyzes the oxidation of oxaloacetate to produce phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to produce NAD+ in turn, The rate of NADH decline was measured at 340 nm, which reflects PEPCK activity.

• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.