Human IL-3 Protein
Human IL-3 Protein, expressed in E. coli
Product Specifications
Background
IL-3 (interleukin 3), also known as IL3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency.
Product Name Alternative
IL-3 Protein, Human; Interleukin-3 Protein, Human; MCGF Protein, Human; MULTI-CSF Protein, Human
Gene ID
3562
Accession Number
P08700
Tag
IL-3
Type
Cytokines and proteins
Applications
No tag
Purity
> 98 % as determined by SDS-PAGE
Activity
Measured in a cell proliferation assay using TF-1. The ED50 for this effect is typically 0.132 ng/mL. The specific activity of recombinant Human IL3 is approximately >7.58 x 106 IU/mg.
Molecular Weight
15.2 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human IL-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Human IL-3 consists of 134 amino acids and has a predicted molecular mass of 15.2 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Applications Notes
Human
Other Product Names
IL-3 Protein; Interleukin-3 Protein; MCGF Protein; MULTI-CSF Protein
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/P08700
AA Sequence
Amino acid sequence derived from Human IL-3 (NP_000579.2) (Ala20-Phe152) was expressed with an initial Met.
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