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X-Gluc (cyclohexanamine)

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator) . X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue) [1].

Product Specifications

CAS Number

[114162-64-0]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye; β-glucuronidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/x-gluc-cyclohexanamine.html

Concentration

10mM

Purity

99.96

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

NC1CCCCC1.O[C@H]([C@@H](O)[C@@H]2O)[C@@H](O[C@@H]2C(O)=O)OC3=CNC4=C3C(Cl)=C(Br)C=C4

Molecular Formula

C20H26BrClN2O7

Molecular Weight

521.79

Precautions

H302, H315, H319, H335

References & Citations

[1]Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51 (5) :402-404.

Shipping Conditions

Room Temperature

Storage Conditions

4°C (Powder, protect from light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Available Sizes

Curated Selection

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