T5 Exonuclease
Recombinant T5 Exonuclease
Product Specifications
Product Name Alternative
T5 Exonuclease
Source
Escherichia Coli
Purity
Greater than 95% as determined by SDS-PAGE.
Form
Sterile Filtered colorless solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Gibson Assembly. Unit Definition: 1 unit of T5 Exonuclease is defined as the amount of enzyme required to cause the change of 0.00032 A260nm/min at 37° C in 1xReaction Buffer: 20mM Tris-acetate (pH 7.9 @ 25°C), 50mM Potassium Acetate, 10mM Magnesium Acetate and 1mM DTT
Preservative
10U/ul, 50mM Tris-HCl (25°C, pH 7.5), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% glycerol.
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