UNG Active
Recombinant E.Coli Uracil DNA Glycosylase, Active
Product Specifications
Product Name Alternative
UDG, b2580, JW2564, EC 3.2.2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2.2, HIGM4, UNG2.
Source
Escherichia Coli
Field of Research
Epigenetics & Chromatin
Purity
Greater than 97.0% as determined by SDS-PAGE.
Form
Sterile Filtered clear solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Treatment of 0.1μg of uracil containing DNA with 1U UDG for 10 min. at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform. Unit Definition: 1 unit is defined as the amount of enzyme that catalyzes the release of 60pmol of uracil/minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50μl reaction containing 0.2μg DNA (104-105 cpm/μg) in 30 min. at 37°C
Preservative
UNG protein solution (5U/ul) containing 10mM Tris-HCl (25°C, pH 7.4), 50mM KCl, 0.1 mM EDTA, 1mM DTT, 0.1mg/ml rAlbumin & 50% glycerol.
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