Recombinant Human RSPO1

Product Specifications

Product Name Alternative

RSPO1, R-spondin1, RP11-566C13.1, CRISTIN3, FLJ40906, RSPO Rspo1, R-spondin, Rspondin, RP23-325M14.2, Roof plate-specific spondin-1

UniProt

Q2MKA7

Expression Region

21-263

Host

Mammalian Cells

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10ng/ml.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

40 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.