Recombinant Human Tumor Necrosis Factor-beta/TNFSF1
Recombinant Human Tumor Necrosis Factor-beta/TNFSF1
Product Specifications
Source
Escherichia coli
Field of Research
Cancer Biology; Immunology & Inflammation
Endotoxin
Less than 0.1 EU/ug of rHuTNF-β/TNFSF1 as determined by LAL method.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 x108 IU/mg in the presence of actinomycin D.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
Molecular Weight
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 171 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L
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