Recombinant Human Leukemia Inhibitory Factor
Recombinant Human Leukemia Inhibitory Factor
Product Specifications
Product Name Alternative
Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor, MLPLI
UniProt
P15018
Source
Escherichia coli
Field of Research
Cancer Biology
Endotoxin
Less than 1 EU/ug of rHuLIF as determined by LAL method.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
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