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Recombinant Human Leukemia Inhibitory Factor

Recombinant Human Leukemia Inhibitory Factor

Product Specifications

Product Name Alternative

Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor, MLPLI

UniProt

P15018

Source

Escherichia coli

Field of Research

Cancer Biology

Endotoxin

Less than 1 EU/ug of rHuLIF as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Available Sizes

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