Recombinant Human Acidic Fibroblast Growth Factor, 2-155a.a.
Recombinant Human Acidic Fibroblast Growth Factor, 2-155a.a.
Product Specifications
UniProt
P05230
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 0.1 EU/ug of rHuaFGF, 2-155a.a. as determined by LAL method.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg in the presence of 10 μg/ml of heparin.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 2 mM EDTA, 0.5 mM DTT, 5 % Trehalose.
Molecular Weight
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD
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