Recombinant Human Fibroblast Growth Factor 12

Product Specifications

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 0.1 EU/ug of rHuFGF-12 as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

The biological activity was determined by its binding ability in a functional ELISA. Immobilized rHuFGF R4/Fc Chimera at 5 μg/mL (100 μL/well) can bind rHuFGF-12 with a linear range of 1.6-100 ng/ml.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 1 mM DTT.

Molecular Weight

Approximately 20.5 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T