Recombinant Human Platelet-derived Growth Factor-AA
Recombinant Human Platelet-derived Growth Factor-AA
Product Specifications
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 0.1 EU/ug of rHuPDGF-AA as determined by LAL method.
Purity
> 95 % by SDS-PAGE.
Bioactivity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50--200 ng/mL in a fluorometric assay using the redox sensitive dye.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Molecular Weight
Approximately 28.8 kDa, a disulfide-linked homodimeric protein containing two 126 amino acid residues polypeptide. But it migrates with an apparent molecular mass of 33.6 kDa in SDS-PAGE.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
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