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Recombinant Human Sonic Hedgehog N-Terminus

Recombinant Human Sonic Hedgehog N-Terminus

Product Specifications

Product Name Alternative

HHG-1

UniProt

Q15465

Source

Escherichia coli

Endotoxin

Less than 1 EU/ug of rHuSHH as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x103 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Molecular Weight

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Available Sizes

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