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Recombinant Human Melanoma Inhibitory Activity Protein

Recombinant Human Melanoma Inhibitory Activity Protein

Product Specifications

UniProt

Q16674

Source

Escherichia coli

Field of Research

Cancer Biology

Endotoxin

Less than 0.1 EU/ug of rHuMIA as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.

Molecular Weight

Approximately 12.1 kDa, a single non-glycosylated polypeptide chain containing 107 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ

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