Recombinant Human Osteopontin/OPN

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37 ̊C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.

Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.