Recombinant Human Glial Cell Line-derived Neurotrophic Factor
Recombinant Human Glial Cell Line-derived Neurotrophic Factor
Product Specifications
Product Name Alternative
ATF, ATF-1
UniProt
P39905
Source
Escherichia coli
Field of Research
Neuroscience
Endotoxin
Less than 0.1 EU/ug of rHuGDNF as determined by LAL method.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 x 107 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, with 0.05 % Tween-20.
Molecular Weight
Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
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