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Recombinant Human Noggin, Insect Cells Derived

Recombinant Human Noggin, Insect Cells Derived

Product Specifications

UniProt

Q13253

Source

Insect Cell

Endotoxin

Less than 0.1 EU/ug of rHuNoggin, Insect Cell as determined by LAL method.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose, 0.02%Tween-20.

Molecular Weight

Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa) .

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC

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