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Recombinant Human Growth Differentiation Factor 6/Bone Morphogenetic Protein-13

Recombinant Human Growth Differentiation Factor 6/Bone Morphogenetic Protein-13

Product Specifications

UniProt

Q6KF10

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

Endotoxin

Less than 0.1 EU/ug of rHuGDF-6/BMP-13 as determined by LAL method.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 2.0 μg/ml, corresponding to a specific activity of > 500 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Molecular Weight

Approximately 27.1 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR

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