Recombinant Rhesus Macaque Tumor Necrosis Factor-alpha/TNFSF2
Recombinant Rhesus Macaque Tumor Necrosis Factor-alpha/TNFSF2
Product Specifications
Product Name Alternative
TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin
UniProt
P48094
Source
Escherichia coli
Field of Research
Cancer Biology; Immunology & Inflammation
Endotoxin
Less than 1 EU/ug of rRhTNF-α as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg in the presence of actinomycin D.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
Molecular Weight
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
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