Recombinant Murine Macrophage Colony Stimulating Factor

Product Specifications

Product Name Alternative

CSF-1, MGI-IM

UniProt

Q3U4F9

Source

Escherichia coli

Field of Research

Immunology & Inflammation

Endotoxin

Less than 1 EU/ug of rMuM-CSF as determined by LAL method.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg.

Form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.

Molecular Weight

Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE