Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
Product Specifications
UniProt
P50592
Source
Escherichia coli
Field of Research
Immunology & Inflammation; Cell Biology
Endotoxin
Less than 0.1 EU/ug of rMuTRAIL/TNFSF10 as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg in the presence of actinomycin D.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT.
Molecular Weight
Approximately 20.2 kDa, a single non-glycosylated polypeptide chain containing 175 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN
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