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Recombinant Murine Acidic Fibroblast Growth Factor

Recombinant Murine Acidic Fibroblast Growth Factor

Product Specifications

Product Name Alternative

FGF-1, HBGF-1

UniProt

P61148

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rMuaFGF as determined by LAL method.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x106 IU/mg in the presence of 10 μg/ml of heparin.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD

Available Sizes

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