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Recombinant Murine Fibroblast Growth Factor 8

Recombinant Murine Fibroblast Growth Factor 8

Product Specifications

Product Name Alternative

AIGF, HBGF-8, FGF-8c

UniProt

P37237

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rMuFGF-8 as determined by LAL method.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x105 IU/mg in the presence of 10 μg/ml of heparin.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.

Molecular Weight

Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR

Available Sizes

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