Recombinant Murine Keratinocyte Growth Factor-2/FGF-10
Recombinant Murine Keratinocyte Growth Factor-2/FGF-10
Product Specifications
UniProt
O35565
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 1 EU/ug of rMuKGF-2/FGF-10 as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol.
Molecular Weight
Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT
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