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Recombinant Murine Fibroblast Growth Factor 21

Recombinant Murine Fibroblast Growth Factor 21

Product Specifications

UniProt

Q9JJN1

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rMuFGF-21 as determined by LAL method.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 3 x PBS, pH 7.4.

Molecular Weight

Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS

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