Recombinant Murine Fibroblast Growth Factor 21
Recombinant Murine Fibroblast Growth Factor 21
Product Specifications
UniProt
Q9JJN1
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 1 EU/ug of rMuFGF-21 as determined by LAL method.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 3 x PBS, pH 7.4.
Molecular Weight
Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS
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