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Recombinant Murine Vascular Endothelial Growth Factor 164, Yeast

Recombinant Murine Vascular Endothelial Growth Factor 164, Yeast

Product Specifications

Source

Yeast

Field of Research

Infectious Disease & Virology; Cardiovascular Research; Stem Cell & Developmental Biology

Endotoxin

Less than 0.01 EU/ug of rMuVEGF164, Yeast as determined by LAL method.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is between 1.0-5.0 ng/ml.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from approximately 40 kDa in SDS-PAGE under non-reducing conditions.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Available Sizes

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