Recombinant Murine Glial Cell Line-derived Neurotrophic Factor
Recombinant Murine Glial Cell Line-derived Neurotrophic Factor
Product Specifications
UniProt
P48540
Source
Escherichia coli
Field of Research
Neuroscience
Endotoxin
Less than 0.1 EU/ug of rMuGDNF as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x106 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.05 % Tween-20.
Molecular Weight
Approximately 29.9 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI
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