Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
Product Specifications
UniProt
P43029
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research
Endotoxin
Less than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Molecular Weight
Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
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