Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2
Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2
Product Specifications
Product Name Alternative
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin
UniProt
P16599
Source
Escherichia coli
Field of Research
Cancer Biology; Immunology & Inflammation
Endotoxin
Less than 1 EU/ug of rRtTNF-α as determined by LAL method.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg in the presence of actinomycin D.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.
Molecular Weight
Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL
Available Sizes
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