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Recombinant Rat Keratinocyte Growth Factor-2/FGF-10

Recombinant Rat Keratinocyte Growth Factor-2/FGF-10

Product Specifications

UniProt

P70492

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rRtKGF-2/FGF-10 as determined by LAL method.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 x103 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.

Molecular Weight

Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

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