Recombinant Rat Keratinocyte Growth Factor-2/FGF-10
Recombinant Rat Keratinocyte Growth Factor-2/FGF-10
Product Specifications
UniProt
P70492
Source
Escherichia coli
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 1 EU/ug of rRtKGF-2/FGF-10 as determined by LAL method.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 x103 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.
Molecular Weight
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
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