Recombinant Rat Heparin-binding EGF-like Growth Factor
Recombinant Rat Heparin-binding EGF-like Growth Factor
Product Specifications
Product Name Alternative
HBEGF, DT-R
UniProt
Q06175
Source
Escherichia coli
Field of Research
Cancer Biology; Stem Cell & Developmental Biology
Endotoxin
Less than 1 EU/ug of rRtHB-EGF as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
Molecular Weight
Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
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