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Recombinant Rat Platelet-derived Growth Factor-BB

Recombinant Rat Platelet-derived Growth Factor-BB

Product Specifications

UniProt

Q05028

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 0.1 EU/ug of rRtPDGF-BB as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 6.9.

Molecular Weight

Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain) .

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

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