Recombinant Rat Ciliary Neurotrophic Factor
Recombinant Rat Ciliary Neurotrophic Factor
Product Specifications
Product Name Alternative
CNTF
UniProt
P20294
Source
Escherichia coli
Endotoxin
Less than 1 EU/ug of rRtCNTF as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.
Molecular Weight
Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
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