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Recombinant Rat Ciliary Neurotrophic Factor

Recombinant Rat Ciliary Neurotrophic Factor

Product Specifications

Product Name Alternative

CNTF

UniProt

P20294

Source

Escherichia coli

Endotoxin

Less than 1 EU/ug of rRtCNTF as determined by LAL method.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.

Molecular Weight

Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Available Sizes

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