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Recombinant Rat Glial Cell-derived Neurotrophic Factor

Recombinant Rat Glial Cell-derived Neurotrophic Factor

Product Specifications

Product Name Alternative

ATF

UniProt

Q07731

Source

Escherichia coli

Field of Research

Neuroscience

Endotoxin

Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x106 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4.

Molecular Weight

Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

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