Recombinant Bovine Basic Fibroblast Growth Factor

Product Specifications

Product Name Alternative

FGF-2, HBGF-2

UniProt

P03969

Source

Escherichia coli

Field of Research

Neuroscience; Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rBobFGF as determined by LAL method.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS