Recombinant Equine Interleukin-1 Receptor Antagonist Protein
Recombinant Equine Interleukin-1 Receptor Antagonist Protein
Product Specifications
Product Name Alternative
IL-1RN, IRAP
UniProt
O18999
Source
Escherichia coli
Field of Research
Immunology & Inflammation; Pharmacology & Drug Discovery
Endotoxin
Less than 1 EU/ug of rEqIL-1RA as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ
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