Recombinant Human P53
Recombinant Human P53
Product Specifications
Product Name Alternative
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
UniProt
P04637
Expression Region
7-393
Reactivity
Human
Tag
N-terminal His-EGFP Tag
Source
E.Coli
Field of Research
Cancer Biology
Purity
Greater than 95% as determined by SDS-PAGE
Molecular Weight
74 kDa
Storage Conditions
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Notes
For research use only.
Applications Notes
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Tested Applications
ELISA, WB
Preservative
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Available Sizes
Frequently Asked Questions
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