Prestained Protein Ladder (25-400 kDa)
This Prestained Protein Ladder (25-400 kDa) is suitable for WB. It is provided as liquid in 67 mM Tris-H3PO4, 5mM EDTA, 2 % SDS, 33 % Glycerol, 0.02 % ProClin300, pH7.5.
Product Specifications
Origin
E. coli
Field of Research
Molecular Biology
Form
Liquid
Storage Conditions
Store at -20°C for one year.
Notes
For research use only.
Applications Notes
1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil! 2. Mix gently, but thoroughly, to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on an SDS-polyacrylamide gel: - 5 μL per well for mini gel, - 10 μL per well for large gel. Use the same volumes for Western blotting. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.
Tested Applications
WB
Preservative
67 mM Tris-H3PO4, 5mM EDTA, 2 % SDS, 33 % Glycerol, 0.02 % ProClin300, pH7.5
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