Prestained Protein Ladder (25-400 kDa)

1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil! 2. Mix gently, but thoroughly, to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on an SDS-polyacrylamide gel: - 5 μL per well for mini gel, - 10 μL per well for large gel. Use the same volumes for Western blotting. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.