TEV Protease

TEV Protease is a highly sequence-specific cysteine protease that is found in the Tobacco Etch Virus (TEV) . The TEV Protease recognition sequence is Glu-Asn-Leu-Tyr-Phe-Gln- (Ser) [ENLYFQ (S) ] and cleavage occurs between the Gln and Ser residues. The amino acid in the P1' position can also be G, A, M, C, or H. TEV Protease is used to cleave affinity tags from fusion proteins. The protease has a His-tag for easy removal from a reaction using nickel affinity resins.