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RSPO1/R-spondin-1, Human (HEK293, His)

Selected Variant

HY-P72784-01

Size: 2 µg

RSPO1/R-spondin-1 is a secreted glycoprotein. RSPO1/R-spondin-1 binds to LGR4/5/6 receptors and ZNRF3/E3 ubiquitin ligases, inhibits Wnt receptor degradation, stabilizes β-catenin and promotes its nuclear translocation, and activates target gene transcription. RSPO1/R-spondin-1, Human promotes intestinal organoid survival, induces pancreatic β-cell proliferation to improve diabetes, enhances osteogenic differentiation of mesenchymal stem cells to treat osteoporosis, and inhibits bone erosion in arthritis models. RSPO1/R-spondin-1 Protein, Human (HEK293, His) is a recombinant RSPO1/R-spondin-1 protein expressed by HEK293 with a C-His tag.

Product Specifications

Product Name Alternative

RSPO1/R-spondin-1 Protein, Human (HEK293, His), Human, HEK293

UNSPSC

12352202

Type

Recombinant Proteins

Assay Protocol

https://www.medchemexpress.com/cytokines/rspo1-protein-human-hek-293-his.html

Purity

98.0

Smiles

SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA

Molecular Formula

284654 (Gene_ID) Q2MKA7-1 (S21-A263) (Accession)

Molecular Weight

Approximately 40-43.3 kDa, based on SDS-PAGE under reducing conditions.

References & Citations

[1]Qu M, et al. Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration. Cell Res. 2021 Mar;31 (3) :259-271.|[2]Levin G, et al. R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and Survival in vitro and Improves Tissue-Engineered Small Intestine Formation in vivo. Front Bioeng Biotechnol. 2020 Jun 5;8:476. |[3]Dossena M, et al. Standardized GMP-compliant scalable production of human pancreas organoids. Stem Cell Res Ther. 2020 Mar 4;11 (1) :94.|[4]Levin G, et al. Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells. BMC Biotechnol. 2020 Jan 20;20 (1) :5.|[5]Silvano S, et al. RSPO1, a potent inducer of pancreatic β cell neogenesis. Cell Rep Med. 2025 May 20;6 (5) :102126. |[6]Wang H, et al. R-Spondin 1 promotes vibration-induced bone formation in mouse models of osteoporosis. J Mol Med (Berl) . 2013 Dec;91 (12) :1421-9.|[7]Krönke G, et al. R-spondin 1 protects against inflammatory bone damage during murine arthritis by modulating the Wnt pathway. Arthritis Rheum. 2010 Aug;62 (8) :2303-12.

Shipping Conditions

Room temperature in continental US; may vary elsewhere.

Storage Conditions

Stored at -20°C for 2 years

Scientific Category

Recombinant Proteins

Available Sizes

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