Klenow Fragment
Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Product Specifications
Product Name Alternative
Large Fragment of DNA Polymerase I, recombinant, E. coli
Concentration
5 units/μl
Purity
The enzyme is greater than 98% pure as indicated by SDS-
Form
Liquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)
Storage Conditions
Store at -20 °C. avoid freeze/thaw cycles
Notes
For research use only.
Applications Notes
Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.
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