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Recombinant Human Fibroblast Growth Factor 19

Recombinant Human Fibroblast Growth Factor 19

Product Specifications

UniProt

O95750

Source

Escherichia coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1 EU/ug of rHuFGF-19 as determined by LAL method.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 μg/ml can bind rHuFGF-19 with a linear range of 3-200 ng/ml.Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x103 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

Available Sizes

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