Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
Product Specifications
Source
Yeast
Field of Research
Infectious Disease & Virology; Cardiovascular Research; Stem Cell & Developmental Biology
Endotoxin
Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.
Purity
> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Available Sizes
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